Anti-Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1214) (VEGF R2, Flk1, KDR, Re

Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, also known as KDR/FLK-1) is a ~200kD member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation. As with other growth factor receptors, upon ligand binding, VEGFR2 dimerizes and is autophosphorylated on multiple tyrosine residues. These phosphotyrosine sites are involved in regulation of intrinsic tyrosine kinase activity or serve as binding sites for SH2 domains and phosphotyrosine binding (PTB) domains of downstream signaling proteins. Phosphorylation of tyrosine 1214 is reported to play a critical role in regulation of receptor autophosphorylation and stimulation of intrinsic tyrosine kinase catalytic activity. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.35-1ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: NIH3T3 cells transfected with full-length, wild-type human VEGFR2 and Porcine Aortic Endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular domain of the CSF-1 receptor coupled to the transmembrane and cytoplasmic domains of the mouse VEGFR2. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.