Anti-Uracil DNA Glycosylase (UNG)

The human UNG gene encodes both mitochondrial (UNG1) and nuclear (UNG2) forms of uracil-DNA glycosylase (UNG). These forms are generated from transcription from alternative promoters, promoter A and promoter B respectively, and the subsequent use of alternative splicing. UNG is responsible for the removal of uracil from DNA by hydrolysis of the N-glycosidic bond that links the base to the deoxyribose backbone, leaving an abasic site. UNG is a highly conserved enzyme found in many species., , Applications: , Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: ELISA:1:1000, Western Blot: 1:1000-1:2,000., Optimal dilutions to be determined by the researcher. Hybridoma: Hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human UNG protein. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.,