Anti-Tumor Necrosis Factor Receptor 1, soluble (sTNFR1)

Applications:, Suitable for use in Neutralization, ELISA and Western Blot. Other applications not tested. Recommended Dilution: Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human sTNFR1 (0.3ug/ml), a concentration of 0.9-1.1ug/ml is required. ELISA: To detect human sTNFR1 by direct ELISA (using 100ul/well antibody solution) a concentration of at least 0.5ug/ml is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4ng/well of recombinant human sTNFR1. , Western Blot: To detect human sTNFR1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human sTNFR1 is 1.5-3ng/lane, under either reducing or non-reducing conditions. Optimal dilutions to be determined by the researcher. , Storage and Stability:, Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.