Anti-Tumor Necrosis Factor alpha, Low Endotoxin (TNFa, TNF-alpha, Tumor Necrosis Factor Ligand Super

TNF alpha is a 17kD cytokine produced by monocytes, macrophages, neutrophils, NK cells and CD4+ T cells. TNF alpha forms dimers and trimers, and is also found as a 26KD integral membrane protein. Mouse TNF alpha has cytolytic activity against many tumor cell lines, and has an important immuno-regulatory role. Applications: Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry (frozen), and Functional Assays. Other applications not tested. Recommended Dilution: Flow Cytomtery: 1:50-1:100, 10ul labels 10e6 cells in 100ul, ELISA: 1-10ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.