Anti-TRIAD3A (Triad Domain-containing Protein 3, Triad3, E3 Ubiquitin-protein Ligase RNF216, RING Fi

TRIADs are novel zinc finger proteins characterized by the presence of Cysteine rich TRIAD domain consisting of DRIL motif flanked by 2 RING fingers. TRIAD3, a 56kD protein, is a novel member of this family. It contains 4 RLD domains in the centre and a proline-rich domain at the C-terminus. Localized in the cytoplasm, it interacts with RIP and inhibits TNF and IL1-induced NF-kappaB activation pathways. Reports suggest TRIAD3 acts as an E3-ubiquitin-protein ligase and promotes ubiquitination and degradation of TLR4 and TLR9, thereby regulating TLR signaling. It also interacts with HIV type I Vif protein and interferes in the early events of HIV-1 replication. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1-3ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Human brain, heart, kidney, liver, lung, ovary, pancreas, small intestine, skeletal muscle, spleen, or testes. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.