Anti-Transforming Growth Factor beta (TGFb) (PE)

TGF-beta RII is a membrane-bound serine/threonine kinase. Upon ligand binding, TGF-beta RII interacts with TGF-beta RI to form the heteromeric signaling complex that transduces TGF-beta signals. A splice variant of the type II receptor, TGF-beta RIIb, contains a 25aa residue insertion near the N-terminus. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated using human lymphocytes., Optimal dilutions to be determined by the researcher., , Flow Cytometry Protocol:, 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction., 2. After blocking, add 10ul of T8250-12L to 1-2.5x10e5 cells and incubate for 30 minutes at RT., 3. Unbound antibody can be removed by washing the cells twice in Flow Cytometry Staining Buffer (Catalog # F4568-10). Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Human Lyse Buffer., 4. Resuspended the cells in Flow Cytometry Staining Buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with PE-labeled goat IgG antibody. Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for 12 months at 4°C. Freezing R-Phycoerythrin (PE) conjugates will result in a substantial loss of activity. PE conjugates are sensitive to light.