Anti-Transforming Growth Factor alpha (TGFa)

TGF-alpha (transforming growth factor alpha) is a 50aa EGF-related polypeptide expressed in a range of tissues, including skin, pancreas, liver, duodenum, salivary glands, urinary and respiratory systems, and the developing and mature central nervous system (CNS). TGF-alpha is produced by monocytes, keratinocytes and neuronal cells and stimulates the proliferation of a range of epidermal and epithelial cells, through binding to the epidermal growth factor receptor (EGFR). TGF-alpha is mitogenic for multipotent neural progenitors and is involved in astrogliosis. Applications: Suitable for use in Direct ELISA, Immunohistochemistry and Western Blot. Other applications have not been tested. Recommended Dilutions: Western Blot: 1ug/ml, ELISA: 2-8ug/ml, Immunohistochemistry: 15ug/ml, Optimal dilutions to be determined by researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 6 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.