Anti-Transcription Elongation Regulator 1 (TCERG1, CA150

This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Applications: Suitable for use in ELISA, Flow Cytometry, Immunocytochemistry, Immunofluorescence (IC) and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (IC): 2-100ug/ml. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with, 0.1% Triton X-100 in PBS., Western Blot: 0.2-2ug/ml, Flow cytometry: 0.5-2ug/ml. HeLa cells with 2% paraformaldehyde/PBS and then permeabilized in 90% methanol., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.