Anti-TAGLN (Transgelin, SM22, WS3-10, 22kD Actin-binding Protein, WS3-10, Smooth Muscle Protein 22 a

TAGLN (Transgelin, also 22kD Actin­binding protein, WS3­10 and Smooth muscle protein 22 alpha) is a 22­24kD cytosolic member of the calponin (calcium­binding and calmodulin­binding troponin T­like protein) family of molecules. It is expressed in both visceral and vascular smooth muscle, fibroblasts, cardiac myocytes, and potentially in breast duct plus prostate epithelium. TAGLN is associated with the actin stress fibers and appears to both suppress MMP­9 production, and downmodulate Ca++­independent smooth muscle contraction. Human TAGLN is 201 amino acids (aa) in length. It contains one CH/calponin homology domain (aa24­ 137), and an actin­binding calponin­like repeat/CLIK (aa175­200). There is one utilized phosphorylation site at Tyr193, plus two utilized acetylation sites in the N­ terminus. Three potential isoform variants are reported, one that shows a alternative start site at Met111, a second that contains a new start site 10 aa upstream of the standard site, and a third that possesses a 58aa substitution for aa97­201. Full­length human and mouse TAGLN share 97% aa sequence identity. Applications: Suitable for use in Western Blot, Immunohistochemistry, and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Immunohistochemistry: 5­15ug/ml, Immunocytochemistry: 5­15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.