Anti-Snail

SNAIL is 29kD nuclear zinc finger transcriptional repressor that contains an N-terminal basic SNAG domain followed by three classical and one atypical zinc finger domains. During development, SNAIL is required for the establishment of left-right axis asymmetry. It also regulates the transcription of E-cadherin and other genes involved in epithelial-mesenchymal transitions during cancer progression. Human SNAIL shares 88% amino acid sequence identity with mouse and rat SNAIL. Applications: Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml detects Snail in HeLa cells, A549 cells and JEG-3 cells., Chromatin Immunoprecipitation: 5ug/5x10e6 cells. Detects Snail in PMA and calcium ionomycin treated Jurkat human acute T cell leukemia cell line chromatin, E-Cadherin promoter detected by standard PCR., Immunocytochemistry: 5-15ug/ml detects Snail in immersion fixed SK-BR-3 human breast cancer cell line treated with MG132 (proteosome inhibitor) and LiCi (GSK3b inhibitor)., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.