Anti-smgGDS-607 Monoclonal Antibody (Clone 4D6)

SmgGDS is a chaperone protein and guanine nucleotide exchange factor encoded by RAP1GDS1 that regulates the prenylation and trafficking of small GTPases and has a role in oncogenesis. Alternative splicing of the RAP1GDS1 pre-mRNA produces a full-length isoform, smgGDS-607, and a short isoform, smgGDS-558. SmgGDS-607 is composed of 607 residues and contains an N-terminal nuclear export sequence and multiple armadillo repeats, which are essential for binding to small GTPases that contain a C-terminal polybasic region. It is expressed in a variety of tissues and cancer cell lines. SmgGDS-607 binds to newly synthesized, preprenylated small GTPases and traffics them to prenyltransferases (PTases), which catalyze their prenylation, a process that is critical for the membrane localization and subsequent activation of small GTPases. An elevated ratio of smgGDS-607 to smgGDS-558 has been observed in cancer cell lines and is associated with reduced survival in patients with breast cancer. Cayman's smgGDS-607 Monoclonal Antibody can be used for ELISA, immunohistochemistry (IHC), and Western blot (WB) applications. The antibody recognizes a unique region of smgGDS-607 that is not present in smgGDS-558, specifically detecting smgGDS-607 at ~60 kDa from human and African green monkey samples.Synonyms: RAP1GDS1, Rap1 GTPase-GDP Dissociation Stimulator 1. Immunogen: Peptide between residues 122-170 of human smgGDS-607. Clone Designation: 4D6. MW: ~60 kDa. Formulation: (Request formulation change), 500 µg of protein G-purified monoclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Mouse. Isotype: IgG1. Applications: ELISA, IHC, WB. Cross Reactivity: (+) smgGDS-607, (-) smgGDS-558.