Anti-Shc, p66, NT, clone 6D757

The human Src homology and collagen (SHC) gene encodes for three proteins (p46, p52, p66). All three SHCs are known to be signaling adaptors that couple activated growth factor receptors to their signaling pathways. p52 and p46 are translated from the same mRNA using two different initiation codons while p66 is generated from a distinct mRNA. p52 and p46 are expressed in every cell type while p66 expression varies from cell type to cell type. Upon stimulation by tyrosine kinase receptors and cytoplasmic TKs, all three SHCs become phosphorylated. Once phosphorylated, p52, p46 and p66 recruit and form a stable complex with GRB2/SOS. p52 and p46 activate the Ras and MAP kinase pathway, while p66 is involved in a signal transduction pathway that regulates the cellular response to oxidative stress and life span. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 0.5ug/ml for HRP/ECL detection., Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubation buffer. , Optimal dilutions to be determined by the researcher , Positive Control Included: N1225-02: Neuro 2A Cell Lysate, Add 100ul SDS-PAGE sample buffer and incubate at 90°C for 5 minutes. Aliquot and store at -20°C, Western Blot: 20ul/lane (mini gel) for HRP/ECL detection. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.