Anti-Secretagogin (SCGN)

Secretagogin (SCGN) is a 32kD member of the EF-hand family of Ca2+-binding proteins. Secretagogin is expressed in select neurons, as well as neuroendocrine cells and pancreatic islets. Human Secretagogin is 276 amino acids (aa) in length and contains six 35 aa EF-hand domains (aa 12-276). There is one alternative splice variant of Secretagogin that shows a 22 aa substitution for aa 28-276, and which demonstrates no Ca2+-binding ability. Secretagogin is known to circulate, presumably released during cell apoptosis. Over aa 164-276, human Secretagogin is more than 97% aa identical to mouse, porcine and canine Secretagogin. Applications: Suitable for use in Western Blotting. Other applications not tested. Recommended Dilution: Western Blotting: 1ug/ml. Lysates from the mouse pancreatic insulinoma beta cell line beta-TC6 was resolved by SDS-PAGE. Following electrophoresis, proteins were transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml anti-Secretagogin., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.