Anti-ROCK-1 (Rho-associated, coiled-coil containing protein kinase 1, ROCKI)

Anti-ROCK-1 (Rho-associated, coiled-coil containing protein kinase 1, ROCKI)
Artikelnummer Größe Datenblatt Manual SDB Lieferzeit Menge Preis
R2998-02D.20 20 µl - -

1 - 19 Werktage

330,00 €
R2998-02D.100 100 µl - -

1 - 19 Werktage

566,00 €
 
ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream... mehr
Produktinformationen "Anti-ROCK-1 (Rho-associated, coiled-coil containing protein kinase 1, ROCKI)"
ROCK (Rho-associated kinase), a family of serine/threonine kinases, is an important downstream target of GTPase Rho and plays an important role in Rho-mediated signaling. Two isoforms of ROCK have been identified (ROCK1 and ROCK2). ROCK is composed of N-terminal catalytic, coiled-coil and C-terminal PH (pleckstrin homology) domains. The C-terminus of ROCK negatively regulates its kinase activity (1,2). Caspase-3-induced cleavage of ROCK1 and direct cleavage of ROCK2 by granzyme B (grB) activates ROCK and leads to phosphorylation of myosin light chain and inhibition of myosin phosphatase (3). This phosphorylation may account for the mechanism by which Rho regulates cytokinesis, cell motility, cell membrane blebbing during apoptosis and smooth muscle contraction (4-6). Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in TBS, 5% BSA, 0.1% Tween-20 at 4°C with gentle shaking, overnight., Immunoprecipitation: 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Hersteller: United States Biological
Hersteller-Nr: R2998-02D

Eigenschaften

Anwendung: IP, WB
Antikörper-Typ: Monoclonal
Klon: 8A34(C8F7)
Konjugat: No
Wirt: Rabbit
Reaktivität: Human, Monkey, Mouse, Rat
Immunogen: Synthetic peptide (KLH-coupled) corresponding to the central sequence of human ROCK1.

Datenbank Information

Handhabung & Sicherheit

Lagerung: -20°C
Versand: +4°C (International: -20°C)
Achtung
Nur für Forschungszwecke und Laboruntersuchungen: Nicht für die Anwendung im oder am Menschen!
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