Anti-Rab11-FIP3

Proteins of the large Rab GTPase family (see RAB1A, MIM 179508) have regulatory roles in the formation, targeting, and fusion of intracellular transport vesicles. RAB11FIP3 is one of many proteins that interact with and regulate Rab GTPases (Hales et al., 2001 [PubMed 11495908]).[supplied by OMIM, Mar 2008], Protein function: Downstream effector molecule for Rab11 GTPase which acts as a regulator of endocytic trafficking and intracellular ciliogenesis by participating in membrane delivery (PubMed:16148947, PubMed:15601896, PubMed:17628206, PubMed:17394487, PubMed:18511905, PubMed:19327867, PubMed:20026645, PubMed:25673879, PubMed:26258637, PubMed:31204173). Recruited by Rab11 to endosomes where it links Rab11 to dynein motor complex (PubMed:20026645). The functional Rab11-RAB11FIP3-dynein complex then regulates the movement of peripheral sorting endosomes (SE) along microtubule tracks toward the microtubule organizing center/centrosome, generating the endocytic recycling compartment (ERC) during interphase of cell cycle (PubMed:17394487, PubMed:20026645). Facilitates the interaction between dynein and dynactin and activates dynein processivity (PubMed:25035494). Binding with ASAP1 is also needed to regulates the pericentrosomal localization of recycling endosomes. The Rab11-RAB11FIP3 complex is also implicated in the transport and fusion of vesicles derived from recycling endosomes to the cleavage furrow during late cytokinesis and abscission (PubMed:16148947, PubMed:15601896). Also regulates cleavage furrow ingression and abscission together with RACGAP1 (PubMed:18511905). Required for ARF6 recruitment to the cleavage furrow during cytokinesis (PubMed:17628206). Involved in the Rab11-Rabin8-Rab8 ciliogenesis cascade by facilitating the orderly assembly of a ciliary targeting complex containing Rab11, ASAP1, Rabin8/RAB3IP, RAB11FIP3 and ARF4, which direct preciliary vesicle trafficking to mother centriole and ciliogenesis initiation (PubMed:26258637, PubMed:31204173). Also promotes the activity of Rab11 and ASAP1 in the ARF4-dependent Golgi- to-cilia transport of the sensory receptor rhodopsin (PubMed:25673879). Competes with WDR44 for binding to Rab11, which controls intracellular ciliogenesis pathway (PubMed:31204173). May play a role in breast cancer cell motility by regulating actin cytoskeleton (PubMed:19327867). [The UniProt Consortium] Recommended dilutions: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.. Cellular localization: Recycling endosome membrane , Peripheral membrane protein. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cleavage furrow . Midbody . In early mitosis remains diffuse and distributed through the cell. The onset of anaphase sequesters these vesicles to the centrosomes at the opposite poles of the cell. During telophase these vesicles move from the centrosomes, to the furrow, and then to the midbody to aid in abscission (PubMed:15158446, PubMed:15601896, PubMed:18511905). Interaction with Rab11 mediates localization to endosomes (PubMed:11495908). Interaction with ARF6 mediates localization to the midbody (PubMed:16148947). .