Anti-Protein Kinase C, alpha

Protein kinase C ('PKC', EC 2.7.11.13) is a cyclic nucleotide-independent enzyme that phosphorylates serine and threonine residues in many target proteins. The PKC family has been divided into three groups differing in the enzymes' cofactor requirements: conventional (c)PKC isoforms (comprising alpha, betaI (also known as beta1), betaII (also known as beta2) and gamma), that require diacylglycerol (DAG), Ca2+, and phospholipid for activation, novel (n)PKC isoforms (comprising delta, epsilon, eta (also known as PKC-L), theta and µ (the mouse homolog of human PKCµ is known as PKD)) that require DAG but not Ca2+, and atypical (a)PKC isoforms, namely zeta, iota and lambda (the mouse homolog of human PKC iota) that require neither Ca2+ nor DAG. A new PKC member has recently been discovered and is referred to as PKCnu., PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells., PKCalpha (also known as PRKCA, PKC alpha) consists of 672 amino acids and is expressed ubiquitously. PKC alpha has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoints, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca2+ handling in myocytes. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05-0.2ug/ml, Western Blot: 0.1-1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.