Anti-PROTEIN A, Biotin Conjugated

Anti-Protein A is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Protein A [Staphylococcus aureus].  Cross reactivity against Protein A from other sources is unknown. Biotin is widely used throughout the biotechnology industry to conjugate proteins for biochemical assays. Biotin's small size typically does not affect the biological activity of protein upon biotinylation. Biotinylated proteins of interest can be enriched from a sample due to highly stable interactions. Biotin conjugated anti-Protein A antibodies are used as an amplifying reagent in immunohistochemistry, microarray assays, ELISAs, blots, and other applications.