Anti-Proopiomelanocortin (Pro-opiomelanocortin, POC, POMC, Adrenocorticotropin, Adrenocorticotropin

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used, adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. Applications: Suitable for use in ELISA, Immunohistochemistry and Western Blot. Other Applications not tested. Recommended Dilutions: Peptide ELISA: 1:128,000, Immunohistochemistry (paraffin): 2-4ug/ml. Requires HIER using sodium citrate buffer pH6.0. Shows vesilulate staining in glandular cells of paraffin embedded human anterior pituitary gland, Western Blot: 0.1-0.3ug/ml detects a band at ~29kD in lysates of cell line NIH3T3. Appears to react with the full-length precursor protein only., Optimal dilutions to be determined by the researcher, Positive Control: Mouse pituitary cell line NIH3T3 lysates, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.