Anti-Presenilin 1

Presenilin 1 (PS1) and presenilin 2 (PS2) are catalytic subunits of the gamma-secretase complex, an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta-amyloid precursor protein). The other members of the gamma-secretase complex are required to have protease activity. PS1 and PS2 may play a role in intracellular signaling and gene expression or in linking chromatin to the nuclear membrane. It regulates epithelial-cadherin function. Three causative genes have been identified that when mutated lead to presenile Alzheimer's disease: APP (amyloid precursor protein gene), PSEN1, and PSEN2. The presenilin 1 gene has recently been discovered on chromosome 14 and is responsible for an early onset form of Alzheimer's disease that accounts for 70-80% of the inherited forms of Alzheimer's. It is a membrane protein that has a region where at least seven mutations have been identified in family members with Alzheimer's disease. Endogenous presenilin mainly exists in a heterodimeric complex formed from the endoproteolytically processed amino-terminal (28 kD) and carboxy-terminal (?17 kD) fragments. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.05-0.2ug/ml, Western Blot: 0.5-2.0ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.