Anti-PRCP (Angiotensinase C, HUMPCP, Prolylcarboxypeptidase, PCP, Lysosomal Pro-X Carboxypeptidase,

PRCP (Prolylcarboxypeptidase, also PCP, lysosomal Pro­X carboxypeptidase and lysosomal carboxypeptidase C) is a lysosomal 57­62kD glycoprotein member of the S28 family of proteases. It is expressed by a number of cells, including fibroblasts, macrophages, and endothelial cells. PRCP cleaves a variety of single C­terminal amino acids (aa) adjacent to a Pro residue, and is known to act on, and inactivate, peptides such as prekallikrein, alpha­MSH and angiotensin II plus III. Human PRCP is apparently synthesized as a prepropeptide that contains a signal sequence (aa1­21), a short prosequence (aa22­45) and a 451aa mature region (aa46­496). The mature region demonstrates a Ser­carboxypeptidase domain (aa55­477) that, in itself, possesses an SKS domain that caps the enzyme's catalytic site. The molecule is known to be mannosylated, and to form noncovalent homodimers. There is one splice variant that contains a 21 aa insertion after Lys56. Over aa1­496, human and mouse PRCP share 77% aa sequence identity. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.