Anti-Plasminogen Activator Inhibitor Type 1 (SERPINE1, PAI, PAI1, PAI-1, PLANH1, serine (or cysteine

Alternate Name: SERPINE1, PAI, PAI1, PAI-1, PLANH1, serine (or cysteine) proteinase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1, plasminogen activator inhibitor, type I, Domain Information: , Gene Ontology Terms: blood coagulation, extracellular region, plasminogen activator activity, serine-type endopeptidase inhibitor activity, Application(s): Peptide ELISA: antibody detection limit dilution 1:32,000. Western Blot: Approx 45kD band observed in Human Duodenum, Liver and Lung lysates (calculated MW of 45.1kD according to NP_000593.1). Recommended concentration: 0.1-0.3ug/ml. 0.1ug/ml staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See P4256-31K-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.