Anti-Obestatin (Appetite-regulating Hormone, Growth Hormone Secretagogue, Growth Hormone-releasing P

Control of appetite involves a number of gastrointestinal hormones that belong to the ghrelin-motilin ligand family that either stimulate food intake and gastric emptying or inhibit these responses. Recently, a new member of the ghrelin-motilin family was identified by using bioinformatic predictions about enzyme cleavage of the prepropeptide of ghrelin. The newly identified 23aa, ghrelin-associated peptide was named obestatin. Although both peptides originate from the same precursor prepropeptide, they have opposing physiological roles. Obestatin exerts anorexigenic effects by decreasing food intake, gastric emptying, jejunal motility, and weight gain by binding to GPR39 orphan receptor, while ghrelin exerts opposing orexigenic effects by binding to growth hormone secretagogue receptor. , Obestatin rat: 23aa, mouse: 23aa, human: 23aa - 2.5kD, human Chromosome: 3p26-p25. Highly expressed in stomach and found in serum as well. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilutions: ELISA: 1:100,000, Western Blot: 1-10ug/ml (ECL), Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.