Anti-NFkB, p65 (Transcription Factor p65, Nuclear Factor NF-kappa-B p65 Subunit, Nuclear Factor of k

RelA belongs to a family of transcription factors (NFKB) that play a fundamental role in inflammatory and immune responses. The NFKB complex is composed of a heterodimer of a Rel family member (RelA, c-Rel, RelB) and either NFKB1 or NFKB2 subunits. RelA and NFKB1 are the most common heterodimeric pair. The NFKB complex is sequestered in the cytoplasm by inhibitory IKB proteins. Upon cellular activation, the ubiquitin-proteosome pathway degrades the IKB proteins allowing the NFKB complex to translocate to the nucleus and activate gene transcription. Applications: Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunoprecipitation: 5ug labels 5 x10e6 cells, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.