Anti-NB-2 (Contactin-5, Cntn5, Neural Recognition Molecule NB-2)

Contactin-5 (UniProt P68500, also known as Neural recognition molecule NB-2) is encoded by the Cntn5 gene (Gene ID 244682) in murine species. Contactin-5 (NB-2) is a glycosylphosphatidylinositol (GPI)-anchored glycoprotein belonging to a subgroup of the immunoglobulin superfamily of proteins known as contactins. Five of the six contactins, Contactin-1, contactin-2/TAG-1, contactin-3/BIG-1, contactin-4/BIG-2, and contactin-5/NB-2 are expressed by DRG neurons. Of these, contactin-1, TAG-1, and BIG-2, are also expressed by motor neurons. NB-2 plays an important role in synapse formation in the developing auditory system in rodents. Expression of NB-2 is seen in the central auditory pathways just before the onset of hearing in rodents and is localized at glutamatergic synapses in the postnatal auditory brainstem. NB-2-knockout mice exhibit abnormal responses to auditory stimuli in both neuronal excitability and behavior, and have delayed auditory brainstem response waves. In addition, NB-2 deficiency in mice causes a significant reduction in the number of synapses and induces apoptosis in a subset of neurons in the auditory brainstem. NB-2 is produced with an N-terminal signal peptide (aa1-23) and a C-terminal propeptide (aa1072-1098) sequence, the removal of which yields the mature GPI-anchored glycoprotein (aa24-1071) with six C2-type Ig-like domains (aa98-659) and four type-III fibronectin domains (aa672-1066). Applications: Suitable for use in Immunofluorescence, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilution: Western Blot: 2ug/mL detects NB-2 in postnatal P14 and adult mouse brain tissue lysates., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.