Anti-Mouse IgG DyLight(TM) 680, clone eB144, DyLight(TM) 680 Conjugated (Fluorescent TrueBlot®)

Fluorescent Mouse TrueBlot(R) Antibody DyLight(TM) 680 Conjugate was prepared from tissue culture supernatant by Protein G affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against native Mouse IgG by both Western blot and ELISA. Mouse IgG TrueBlot(R) is a unique DyLight(TM) 680 conjugated anti-mouse IgG immunoblotting (second step) reagent. Mouse IgG TrueBlot(R) enables detection of immunoblotted target protein bands, without hindrance by interfering immunoprecipitating immunoglobulin heavy and light chains. It is easy to generate publication-quality IP/Fluorescent Western Blot data with Mouse IgG TrueBlot(R), simply substitute the conventional DL680 anti-mouse IgG blotting reagent with Fluorescent Mouse TrueBlot(R) Antibody DyLight(TM) 680 and follow the prescribed protocol for sample preparation and immunoblotting. Mouse IgG TrueBlot(R) is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot(R) with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in IP/Western blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions. Fluorescent Mouse TrueBlot(R) Antibody DyLight(TM) 680 may also be used for detection in immunoassays that do not employ immunoprecipitation.