Anti-Matrix Metalloproteinase 2 (MMP-2, MMP2, MMP-II, Collagenase IV, 72kD Type IV Collagenase, Coll

Loss of negative growth regulation and high invasive potential are neoplastic traits often associated with abnormal expression of matrix matalloproteinases. MMP-2, the signalling mechanism for cells to begin migration, triggers the movement when it splits laminin-5 and exposes and integrin-binding site on this component of epithelial basement membrane. The changed form of laminin was found in tumors and in tissues being altered. The activated form of MMP2, however, is not found in benign tumors. Thus detection of the enzyme is a possible early indicator of tumor activity. Applications: Suitable for use in ELISA, Western Blot, Immunofluorescence/Immunocytochemistry, Immunoprecipitation and Immunohistochemistry. Other applications not tested. Recommended Dilutions: ELISA: 0.03ug/ml, Western Blot 1-2ug/ml, Immunofluorescence (IC): 1:10-1:2000, Immunohistochemistry (Frozen/paraffin): 2ug/ml, Immunoprecipitation: 1-2ug/100-500ug total protein, Optimal dilutions to be determined by the researcher. Positive Control: Human ulcer and human ovary tissue, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.