Anti-MAP3K7, ID (Mitogen-activated Protein Kinase Kinase Kinase 7, Transforming Growth Factor-beta-a

MEKK7, known as TAK1 for transforming growth factor (TGF) beta-activated kinase-1, is a MLK (MAP3K) type protein kinase which is associated with several signaling pathways. The protein contains a N-terminal protein kinase domain preceeded by a short regulatory motif and followed by a C-terminal extension of unknown function. TAK1 is activated by TGF beta and tumor necrosis factor (TNF). Further, TAK1 binding proteins, TAB-1 and -2, bind to the N-terminal regulatory motif and couple TAK1 to additional upstream signals, including the human X chromosome-linked inhibitor of apoptosis. TAK1 regulates the JNK cascade by phosphorylating MKK4/MAP2K4 and p38 cascades by phosphorylating MEK3/MAP2K3 and MKK6/MAP2K6. TAK1 also has been implicated in interleukin-1 (IL-1) signaling to JNKs via direct activation by IL-1 or by forming a complex with TRAF6, TAB1 and TAB2. At least four protein isoforms, resulting from alternatively spliced transcript variants, have been identified - 491, 518, 579 and 606aa. Applications: Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: ELISA: 1:40,000, Western Blot: 1:500-1:1000, Immunohistochemistry (Formalin fixed paraffin embedded): 5ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.