Anti-Map3k3, ID (Map3k3, Mekk3, Mitogen-activated protein kinase kinase kinase 3, MAPK/ERK kinase ki

Component of a protein kinase signal transduction cascade. Map3k3 mediates activation of the NF-kappa-B, AP1 and DDIT3 transcriptional regulators. Applications: Suitable for use in Western Blot, ELISA, Recommended Dilution: ELISA: 1:1,000, Western Blot: 1:100-500, Storage and Stability: Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. , , Note: Applications are based on unconjugated antibody.