Anti-Mannose Binding Protein (Mannan Binding Protein (MBP, Mannan Binding Lectin, MBL) (FITC)

Mannose Binding Lectin (MBL) also called mannose- or mannan-binding protein (MBP) is a member of the group of collectins. MBL is an oligomeric lectin that recognizes carbohydrates as mannose and N-acetylglucosamine on pathogens. MBL contains a cysteine rich, a collagen like and a carbohydrate recognition domain. It forms a complex with C1r/C1s like serine proteases designated MASPs that proteolytically cleave C4, C2 and C3. MBL is able to activate the complement pathway independent of the classical and alternative complement activation pathways. The MBL-MASP pathway (better known as the lectin pathway) is antibody and C1q-independent. MBL exhibits complement-dependent antibacterial activity and acts directly as an opsonic and therefore plays an important role in innate immunity. MBL is synthesized by hepatocytes and has been isolated from the liver or serum of various vertebrate species. Applications: Suitable for use in Western Blot, FLISA, Immunohistochemistry and Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50, Western Blot: 1:50, Immunohistochemistry (Frozen): 1:50. Not suitable for staining paraffin embedded sections, Optimal dilutions to be determined by the researcher. Storage and Stability: For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. FITC conjugates are sensitive to light.