Anti-LL-37 (Clone 3G10)

LL-37 is a cationic alpha-helical peptide expressed in human bone marrow, testis, granulocytes, gingival epithelium, and a variety of immune cells. It is produced by proteolytic cleavage of the cathelicidin human cationic antimicrobial protein of 18 kDa (hCAP18). LL-37 has antimicrobial and antiviral activity, and protein levels of LL-37 are increased in epithelial cells, macrophages, and neutrophils following bacterial infection in vitro. It functions as a chemoattractant for human monocytes, neutrophils, and T cells, and induces chemokine secretion from epithelial cells in infected tissues. LL-37 is a component of LPS-induced neutrophil extracellular traps (NETs) produced from human neutrophils isolated from patients with systemic lupus erythrematosus (SLE) or individuals without SLE. It also enhances formation of NETs induced by phorbol 12-myristate 13-acetate (PMA) or S. aureus. LL-37 can be citrullinated by protein arginine deiminase 2 (PAD2) and PAD4, a modification that reduces its antibacterial and antiviral activities. Native, but not citrullinated, LL-37 prevents mortality in a mouse model of D-galactosamine-sensitized endotoxic shock. Cayman's LL-37 Monoclonal Antibody (Clone 3G10) can be used for ELISA and flow cytometry (FC) applications.Synonyms: Antibacterial Protein LL-37. Immunogen: Synthetic human LL-37. Clone Designation: 3G10. Formulation: (Request formulation change), 100 µg of protein G-affinity purified monoclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Mouse. Isotype: IgG1. Applications: ELISA, FC. Cross Reactivity: (+) LL-37, (+) hCAP18. UniProt Accession #: P49913.