Anti-LIM homeobox protein 1, 3G3 (LIM/homeobox protein Lhx1, Homeobox protein 1, hLim-1, LHX1, LIM-1

LIM homeobox protein 1 is a potential transcription factor that may play a role in differentiation and development of neural and lymphoid cells. It contains 1 homeobox DNA-binding domain and 2 LIM zinc-binding domains. The latter have a negative regulatory function and disruption of the LIM domains results in an avtivated form. It localises to the nucleus and is expressed in the brain, thymus and tonsils. It is also expressed in smaples from patients with chronic myeloid leukemia and in 58% of acute myeloid leukemia cell lines. Applications: Suitable for use in Western Blot and direct ELISA. Other applications have not been tested. Recommended Dilutions: Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile 40-50% glycerol, ddH2O. Reconstituted product is stable for 12 months at -20°C. Aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.