Anti-Leptin Receptor (OBR, LEPR, B219, Obese Receptor)

Obesity, a common nutritional disorder, is associated with diabetes, hypertension, hyperlipidemia, cancer and many other health related problems. At least five genes, Obese (ob), diabetes (db), fat (fat), agouti yellow (Ay), and tubby (tub) have been linked to obesity. Obese gene encodes an adipocyte-tissue derived secreted protein Ob protein/Leptin (146aa, ~16kD) that controls body weight homeostasis. Leptin mediates its effects via the Leptin Receptor or Obese Receptors (OBR or LEPR) that is expressed in several tissues including hypothalamus. The Ob-R has at least 6 alternatively spliced forms (OBRa-f or LRa-f) that contain a common extracellular domain. Leptin is structurally related to cytokine family and it activates cytokine-like signal transduction by stimulating the JAK-STAT pathway via the OBRb. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1:2000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.