Anti-Lck Interacting Molecule (Lime1, Lck interacting transmembrane adaptor 1, 2810038K19Rik, LIME,

Alternate Name: Lime1, Lck interacting transmembrane adaptor 1, 2810038K19Rik, LIME, RIKEN cDNA 2810038K19 gene, Domain Information: , Gene Ontology Terms: integral to membrane, extracellular space, Application(s): Peptide ELISA: antibody detection limit dilution >1:128,000. Western Blot: Approx 32kD band observed in Mouse Heart lysates (predicted MW of 31kD according to NP_076173.1). Recommended for use at 0.5-2ug/ml. Staining (1ug/ml) of Mouse Heart lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See L1566-04-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.