Anti-Jagged1 (JAG1, AGS, AHD, AWS, HJ1, JAGL1, Alagille Syndrome Protein, CD339, Headturner, Htu, MG

Jagged 1-CFS was the first ligand for Notch identified in mammals. Since both the ligands and the receptors are transmembrane proteins, direct cell-cell interactions are thought to be required for activating Notch signaling. Its large extracellular region has a DSL (Delta, Serrate, Lag-2 consensus sequence) domain followed by 16 EGF-like repeats, and a cysteine-rich (CR) region. The DSL motif is necessary for binding to Notch receptors and the EGF repeats modulate the affinity of the interaction with Notch receptors. Notch signaling is implicated in many developmental processes in a variety of cell types. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation. Some specific areas where Jagged is involved include hematopoiesis, myogenesis, neurogenesis, and development of the vasculature. Furthermore, Jagged 1 mutations are implicated in Alagille syndrome, an autosomal dominant disorder characterized by defects in liver, heart, kidney, eye, skeletal, craniofacial tissues. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul to label 1x10e6 cells. Using mouse endothelioma cell line and rat cortical stem cells, Optimal dilutions to be determined by the researcher. Hybridoma: NSO myeloma cells with B cells from Balb/c mice. Absorption: 488nm , Emission: 515-545nm, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. CFS conjugates are sensitive to light.