Anti-Interleukin-5 (IL-5), clone 4H163

Applications:, ELISA Capture: Useful as a capture antibody in a sandwich ELISA for quantifying mouse or human IL-5 protein levels. Purified antibody should be paired with biotinylated antibody (Cat. No. I8427-10E) as the detection antibody, with purified recombinant mouse IL-5 as the standard., Immunofluorescence/Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-5-positive cells within mixed cell populations. Recombinant mouse IL-5 or Rat IgG1 should be used as a specificity control., Western Blot: The purified antibody is also useful for Western blot applications. For Western blotting, a concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of < 100ng/lane of recombinant mouse IL-5 under reducing conditions. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.