Anti-Interleukin 10R, beta (IL-10 R-beta, Interleukin 10 Receptor beta, IL-10R2, CD210b, CRF2-4)

IL-10 R-beta (Interleukin 10 receptor beta, also IL-10R2, CD210b and CRF2-4) is an 80-85kD member of the type II cytokine receptor family of proteins. It is very widely expressed and serves as a signal transducing accessory chain when complexed to the ligand-binding chains for IL-10, -22, -28A, -28B, and -29 (plus IL-26 in human). Mature mouse IL-10 R-beta is a type I transmembrane protein with an extracellular domain that possesses two fibronectin type III domains (aa22-107 and 11-208). There is one alternate start site at Met20. Applications: Suitable for use in ELISA and Flow Cytometry. Other applications not tested. Recommended Dilutions: ELISA: Direct in Recombinant mouse IL-10Rb, Flow Cytometry: 25ug/ml. 10ul labels 1-2.5x10e5 cells in a total reaction volume <200ul., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.