Anti-Interleukin-10 (IL-10)

Interleukin-10 is produced by a population of T cells. It has a wide range of biological properties, including inhibition of macrophage and T helper cell mediated cytokine synthesis suppression of the delayed hypersensivity response. Reports suggest that IL-10 may initially be involved in the polarization the Th2 responses. Applications: Suitable for use in ELISA, Western Blot and Neutralization. Other applications not tested. Recommended Dilution: ELISA: 0.5ug/ml, Western Blot: 0.1-0.2ug/ml, Neutralization: 0.012-0.014ug/ml to yield one half maximal inhibition (ND50). , Optimal dilutions to be determined by the researcher. Recommended Secondary Reagents: Sheep anti Rabbit IgG: HRP, Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.