Anti-Interferon Regulatory Factor 4 (IRF4, IRF-4, Lymphocyte-specific Interferon Regulatory Factor,

Interferon regulatory factor 4 (IRF4), also known as MUM1 and LSIRF, is a 51kD lymphocyte restricted transcription factor. It is required for immunoglobulin class switching and terminal differentiation of B cells. IRF4 is overexpressed in multiple myeloma and cooperates with Myc in an autoregulatory loop. In T cells, IRF4 is required for the production of IL4. IRF4 contains an N-terminal DNA binding domain that is homologous to that in other IRF proteins. Within the C-terminal domain (aa130-451), human IRF4 shares 90% aa sequence identity with mouse and rat IRF4. Alternate splicing may generate isoforms with N-terminal, C-terminal, or internal deletions. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 0.5ug/ml, Immunohistochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.