Anti-Integrin beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla), clone 0.N.97

CD29 is the integrin B1 chain which is expressed as heterodimer non-covalently associated with the integrin subunits 1 to 6 (CD49a to CD49f) and known as very late antigens (VLA). It is also called platelet GPlla. It has been reported as receptor of ?bronectin. The antigen mediates cellular adhesion primarily to collagen, laminin or fibronectin. It has a broad cellular reactivity but is not expressed by erythrocytes. In normal peripheral blood CD29 blood CD29 is found on approximately 41% of T cells, 40% of CD4+ T lymphocytes, 43% of CD8+ T lymphocytes (1). It is slightly reactive with peripheral B-cells. The co-expression of CD4 and CD29 allows the identi?cation of the helper/inducer subpopulation of CD4 lymphocytes. Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry (frozen only). Not assessed for Western Blotting. , Recommended Dilution: , Immunoprecipitation: Use Protein G, 2ug antibody/mg protein lysate., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.