Anti-Insulin-Like Growth Factor Binding Protein-Related Protein 10 (IGFBP-rP10)

IGFBP-rP10 (IGF binding protein-related protein 10, also Kazal-type serine protease inhibitor domain-contain protein 1 and BONO1) is a 37kD (predicted), secreted member of the IGFBP superfamily. It is expressed by osteoblasts and odontoblasts, is associated with regions of mineralization, and stimulates the proliferation of osteoblasts in vitro. Mature human IGFBP-rP10 is 274aa in length. It contains an IGF binding domain (aa49-124), a follistatin-like serine protease inhibitor region (aa125-168) and a C2-type Ig-like domain (aa172-269). There is one splice variant that shows a 70aa substitution for aa184-304. Mature human IGFBP-rP10 is 87% aa identical to mouse IGFBP-rP10. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml, Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.