Anti-Immunoglobulin-Like Transcript 2 (ILT2, CD85j) (Carboxyfluorescein)

ILT2, also known as LIR1, MIR7, and CD85j, is expressed on most monocytes, dendritic cells, and mature B cells. It is also expressed on small percentages of T cells and NK cells. ILT2 has four extracellular Ig-like domains and three cytoplasmic ITIMs. It functions as an inhibitory receptor that prevents cellular activation. ILT2 has been shown to bind classical (HLA-A and -B) and non-classical (HLA-G1, -E and -F) MHC class I molecules (MHC I). ILT2 also binds with high affinity to an MHC I paralog from human cytomegalovirus. Ligation of ILT2 by MHC I may function to poise cellular activation thresholds and inhibit various leukocyte effector mechanisms that are regulated by MHC I molecules on target cells. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: Use 10ul to label 1x10e5 cells., Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Carboxyfluorescein conjugates are sensitive to light.