Anti-IL22RA2 (Interleukin-22 Receptor Subunit alpha-2, IL-22 Receptor Subunit alpha-2, IL-22R-alpha-

IL22RA2 is a 40kD secreted glycoprotein that belongs to the class II cytokine receptor family. The mature region contains two fibronectin type III domains. IL22RA2 will bind to an IL-22 dimer, rendering it inactive. The amino acid sequence of mature mouse IL22RA2 is 85% and 67% identical to that of rat and human IL22RA2, respectively. Unlike the human IL22RA2 gene that shows three alternate splice forms, the mouse IL22RA2 gene shows only single nucleotide polymorphisms. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 1x10e6 cells. For intracellular staining, cells must first be fixed with 4% paraformaldehyde fixation buffer and permeabilized with 0.1% saponin balanced salt solution., Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Harvest cells and wash twice in saline buffer., 2. Cell surface staining may be done at this point following the manufacturer's staining procedure., 3. Resuspend 5x10e5 cells in 0.5ml of cold 4% paraformldehyde fixation buffer and incubate at RT for 10 mins., 4. Following fixation, wash the cells twice in saline buffer, then once in 0.1% saponin saline buffer., 5. After permeabilization, add 10ul of I8443-21A and incubate the cells for 30 minutes at RT in the dark., 6. Wash the cells twice with 0.1% saponin saline buffer., 7. Resuspend the cells in saline buffer for final flow cytometric analysis. As a control for this analysis, cells in a separate tube should be treated with APC-labeled sheep IgG antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.