Anti-Hsp70 (HspA1A) (Clone 7F6)

Heat shock protein 70s (Hsp70s) are abundant and stress-inducible 70 kDa molecular chaperone proteins encoded by a highly conserved, multigene family. They are monomeric proteins that can be divided into two functional domains: an N-terminal ATPase domain and a substrate binding domain that contains a highly conserved EEVD motif at its C-terminus. Hsp70s are found in the cytosol, nuclei, endoplasmic reticulum, mitochondria, and chloroplasts of eukaryotes, as well as in bacteria. They function as molecular chaperones that assist in a wide range of cellular processes, including refolding of aggregated or misfolded proteins, co- and post-translational folding and assembly of nascent peptides, membrane translocation of secretory and organellar proteins, controlling activity of regulatory nuclear receptors, kinases and transcription factors, as well as cooperativity with the Hsp90 chaperone system in eukaryotes. The Hsp70 chaperone cycle is ATP-dependent and initiated by transient interaction of the Hsp70 substrate binding domain with hydrophobic regions within a peptide or protein. It consists of an alteration between the low-affinity ATP-bound state with fast rates of substrate exchange and the high-affinity ADP bound state with slow rates of substrate exchange. Hsp70s are subject to a variety of post-translational modifications and their expression is upregulated under conditions of cellular stress and in a variety of disease states. Cayman's Hsp70 (HspA1A) Monoclonal Antibody (Clone 7F6) can be used for Western blot, ELISA, immunohistochemistry, and immunoprecipitation applications. The antibody recognizes Hsp70, also known as HspA1A, at ~70 kDa from human and mouse samples.Synonyms: Heat Shock 70 kDa Protein 1A, Heat Shock Protein 70, Hsp70-1. Immunogen: Full length human Hsp70 recombinant protein. Clone Designation: 7F6. Formulation: (Request formulation change), 100 µg protein G-purified monoclonal antibody. Storage Buffer: PBS, pH 7.2, with 50% glycerol and 0.02% sodium azide. Host: Mouse. Isotype: IgG1. Applications: ELISA, IHC, IP, and WB. Cross Reactivity: (-) GRP78.