Anti-Histone H3K79Me3 Monoclonal Antibody (RM157)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Trimethylation of histone H3 at lysine 79 (H3K79Me3) is found at promoter regions of both active and silenced genes in human CD4+ T cells and positively correlates with transcriptional repression. H3K79Me3 is also found at pericentromeric heterochromatin in mouse oocytes and somatic cells. Levels of H3K79Me3 are decreased in human tumor tissue compared to non-cancerous tissue. Cayman's Histone H3K79Me3 Monoclonal Antibody (RM157) can be used for ELISA, multiplex-based assays, and Western blot (WB) applications.Synonyms: Trimethylated Histone H3 Lysine 79. Immunogen: A peptide corresponding to H3K79Me3. Clone Designation: RM157. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, multiplex-based assays, WB. Cross Reactivity: (+) H3K79Me3, (-) Unmodified H3K79, (-) H3K79Me1, (-) H3K79Me2, (-) H3K4Me1, (-) H3K4Me2, (-) H3K4Me3, (-) H3K9Me1, (-) H3K9Me2, (-) H3K9Me3, (-) H3K14Me2, (-) H3K18Me1, (-) H3K18Me2, (-) H3K23Me1, (-) H3K23Me2, (-) H3K27Me1, (-) H3K27Me2, (-) H3K27Me3, (-) H3K36Me1, (-) H3K36Me2, (-) H3K36Me3, (-) H3K56Me1.