Anti-Histone H3K4Me1 Monoclonal Antibody (RM140)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Monomethylation of histone H3 at lysine 4 (H3K4Me1) is found at active and primed enhancer regions of gene promoters and H3K4Me1-containing nucleosomes are more efficiently remodeled by the chromatin-remodeling complex BAF than unmarked nucleosomes. It is also enriched at CpG sites associated with aging in stem and differentiated cells. Cayman's Histone H3K4Me1 Monoclonal Antibody (RM140) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.Synonyms: Monomethylated Histone H3 Lysine 4. Immunogen: Peptide corresponding to H3K4Me1. Clone Designation: RM140. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ChIP, ELISA, ICC, multiplex-based assay, WB. Cross Reactivity: (+) H3K4Me1, (-) Unmodified histone H3 (1-19), (-) H3K4Me2, (-) H3K4Me3, (-) H3K9Me1, (-) H3K9Me2, (-) H3K9Me3, (-) H3K14Me2, (-) H3K18Me1, (-) H3K18Me2, (-) H3K23Me1, (-) H3K23Me2, (-) H3K27Me1, (-) H3K27Me2, (-) H3K27Me3, (-) H3K36Me1, (-) H3K36Me2, (-) H3K36Me3, (-) H3K56Me1, (-) H3K79Me1, (-) H3K79Me2, (-) H3K79Me3.