Anti-Histone H3K4Ac Monoclonal Antibody (RM149)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Acetylation of histone H3 at lysine 4 (H3K4Ac) occurs primarily at the promoter and transcribed regions of active genes. H3K4Ac is enriched at the promoters of genes involved in the estrogen response and epithelial-to-mesenchymal transition (EMT) in MCF-7 and MDA-MB-231 breast cancer cells. Cayman's Histone H3K4Ac Monoclonal Antibody (RM149) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.Synonyms: Acetylated Histone H3 Lysine 4. Immunogen: Peptide corresponding to H3K4Ac. Clone Designation: RM149. Formulation: (Request formulation change), 100 µg of protein A-purified monoclonal antibody. Storage Buffer: PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ChIP, ELISA, ICC, multiplex-based assays, WB. Cross Reactivity: (+) H3K4Ac, (-) Unmodified histone H3 (1-19), (-) H3K9Ac, (-) H3K14Ac, (-) H3K18Ac, (-) H3K23Ac, (-) H3K27Ac, (-) H3K36Ac, (-) H3K56Ac, (-) H3K79Ac, (-) H3K122Ac.