Anti-HGF (Hepatocyte Growth Factor, Hepatopoietin-A, Scatter Factor, SF) (Biotin)

HGF, also known as scatter factor and hepatopoietin A, is a pleiotropic protein in the plasminogen subfamily of S1 peptidases. It is a multidomain molecule that includes an N-terminal PAN/APPLE like domain, four Kringle domains, and a serine proteinase-like domain that has no detectable protease activity. Canine HGF is secreted as an inactive 699aa single chain propeptide. It is cleaved after the fourth Kringle domain by a serine protease to form bioactive disulfide linked HGF with a 60kD A and 30kD B chain. A variant of HGF with a 5aa deletion in the first Kringle domain has been described in canine and other species. Canine HGF shares 97%, 99%, 93%, 93%, and 89% aa sequence identity with bovine, feline, human, mouse, and rat HGF, respectively. HGF binds heparan sulfate proteoglycans and the widely expressed receptor tyrosine kinase, HGR/cMET. HGF dependent cMET activation is implicated in the development of many human cancers. HGF regulates epithelial morphogenesis by inducing cell scattering and branching tubulogenesis. HGF induces the upregulation of integrin A2B1 in epithelial cells by a selective increase in A2 gene transcription. This integrin serves as a collagen I receptor, and its blockade disrupts epithelial cell branching tubulogenesis. HGF can also alter epithelium morphology by the induction of nectin-1a ectodomain shedding, an adhesion protein component of adherens junctions. In the thymus, HGF induces the proliferation, motility, and loss of differentiation markers of thyrocytes and inhibits TSHs-timulated iodine uptake. HGF promotes the motility of cardiac stem cells in damaged myocardium. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 0.1mg/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile Tris, pH 7.3 (20mM Trizma base, 150mM sodium chloride), 0.1% BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.