Anti-HERC1

Protein function: Involved in membrane trafficking via some guanine nucleotide exchange factor (GEF) activity and its ability to bind clathrin. Acts as a GEF for Arf and Rab, by exchanging bound GDP for free GTP. Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity. May also act as a E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. [The UniProt Consortium] Recommended dilutions: WB 1:1000-2000. Cellular localization: Membrane, Peripheral membrane protein. Cytoplasm, cytosol. Golgi apparatus. Recruited onto actin-rich surface protrusions.