Anti-Growth Hormone, human (hGH)

Human growth hormone is a single chain polypeptide hormone essential for growth of all tissues. It is a human serum protein that increases fat mobilization, M.W: 21.7kD. Growth hormone is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene encoding this protein, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same transcriptional orientation, an arrangement which is thought to have evolved by a series of gene duplications. The five genes share a remarkably high degree of sequence identity. Alternative splicing generates additional isoforms of each of the five growth hormones, leading to further diversity and potential for specialization. This particular family member is expressed in the pituitary but not in placental tissue as is the case for the other four genes in the growth hormone locus. Mutations in or deletions of the gene lead to growth hormone deficiency and short stature. Applications: Suitable for use in ELISA, Immunocytochemistry, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1:1000-1:2000, ELISA: 0.1-1ug/ml, Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1906 IgY, Chicken Pab Rb x Ch , I1906-10 IgY, Chicken-HRP Rb x Ch , I1906-12 IgY, Chicken-FITC Rb x Ch , I1906-14 IgY, Chicken-AP Rb x Ch, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.