Anti-Granulocyte Macrophage Colony Stimulating Factor (GM-CSF), clone 3H144

GM-CSF is an extracellular homodimer polyprotein, functioning as a hematopoietic growth factor and immune modulator. It can be produced and act upon a variety of cell types, including T-lymphocytes, B-lymphocytes, monocytes/macrophages, endothelial cells, fibroblasts, stromal cells, mesothelial cells, keratinocytes, osteoblasts, uterine epithelial cells, synoviocytes, mast cells and various solid tumors.  GM-CSF stimulates stem cells to produce granulocytes and monocytes to cope with infection.  Recombinant GM-CSF has been applied to boost white blood cell in cancer patients after chemotherapy and may also be useful as an immune tonic for anemia and AIDS patients. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.